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为了了解树突状细胞 (DC)成熟状态对Glatirameracetate(GA)特异的T细胞分化的影响 ,在体外条件下 ,分析了从多发性硬化症 (MS)病人外周血单个核细胞诱导的成熟与未成熟的树突状细胞对来自多发性硬化症病人的GA特异的T细胞系增殖及细胞因子产生的影响。结果显示 ,体外条件下 ,从MS病人外周血的单个核细胞易于诱导GA抗原特异的T细胞系 (TCL) ;5 μg/ml的脂多糖 (LPS)在 2 4小时内可有效诱导DC的成熟。同未成熟的DC相比 ,成熟的DC更能有效地刺激GA抗原特异的TCL的增殖 ;GA抗原特异的TCL产生高水平的IL 2 ,IL 4 ,IFN γ ,IL 10及低水平的IL 6 ;成熟DC能促进GA抗原特异的TCL的IL 6和IL 10的分泌 ,但下调IL 2 ,IL 4及IFN γ的产生。结论 :DC的成熟状态调控TCL的增殖及细胞因子的产生。
In order to understand the effect of dendritic cell (DC) maturation on Glatirameracetate (GA) -specific T cell differentiation, the in vitro induction of maturation induced by peripheral blood mononuclear cells from patients with multiple sclerosis (MS) Effect of Mature Dendritic Cells on Proliferation and Cytokine Production of GA-Specific T Cell Lines from Patients with Multiple Sclerosis. The results showed that mononuclear cells from peripheral blood of patients with MS could easily induce GA antigen-specific T cell line (TCL) in vitro; 5 μg / ml lipopolysaccharide (LPS) could effectively induce the maturation of DC within 24 hours . Compared with immature DCs, mature DCs were more effective in stimulating GA antigen-specific TCL proliferation; GA antigen-specific TCLs produced high levels of IL 2, IL 4, IFNγ, IL 10, and low levels of IL 6 ; Mature DC can promote the secretion of IL-6 and IL-10 of GA antigen-specific TCL, but down-regulate the production of IL 2, IL 4 and IFNγ. Conclusion: The mature state of DC regulates TCL proliferation and cytokine production.