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目的:通过探究中药钩藤复方对RH大鼠动脉壁形态学及主动脉C1CF含量的影响,进一步探寻钩藤复方逆转高血压血管重构的机制,从而为中药通过逆转VR进而防治高血压靶器官损伤的研究供提理论和实验依据。方法:选取健康雄性Wistar大鼠60只,50只用两肾一夹方式制作RH大鼠模型,其余10只做假手术。将RH大鼠随机分成模型组(M)、钩藤高剂量组(H)、钩藤低剂量组(L)、牛黄降压组(NH)、卡托普利组(CP)5组,每组10只;10只假手术大鼠为空白对照组。造模成功后第4周起,各组大鼠每日一次灌胃给药,持续12周后,取胸主动脉检测中膜胶原面积百分比(VFC)变化和C1CF含量。结果:各给药组与模型组相比,MT、VFC及C1CF含量均不同程度降低,其中钩藤复方高、低剂量组下降最为显著。结论:钩藤复方具有抑制血管重塑的作用,推测可能是通过抑制VSMC的增殖、迁移,从而减少间质分泌和血管壁结构重塑。
OBJECTIVE: To investigate the effect of Houdanfang Compound on arterial wall morphology and aortic C1CF content in RH rats, to further explore the mechanism of Hook-teng compound in reversing vascular remodeling of hypertension and to prevent and cure hypertensive target organ The study of damage provides theoretical and experimental evidence. Methods: Sixty healthy male Wistar rats were selected. Fifty rats were randomly divided into two groups and one kidney was used to make the RH model. The other 10 were sham operated. The RH rats were randomly divided into five groups: model group (M), Uncaria high dose group (H), Uncaria rhynchophylla low dose group (L), Niuhuang hypotensive group (NH) and captopril group 10 rats and 10 sham-operated rats as blank control group. From the 4th week after successful model establishment, the rats in each group were given gavage once a day for 12 weeks, and the changes of the percentage of medial collagenous area (VFC) and C1CF content in the aorta were measured. Results: Compared with the model group, the contents of MT, VFC and C1CF in each drug group decreased to different extents, and the decrease was most significant in the high and low dose groups. Conclusion: Uncaria compound can inhibit vascular remodeling, presumably by inhibiting VSMC proliferation and migration, thereby reducing the interstitial secretion and remodeling of vascular wall structure.