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目的:建立流式微球载体技术(FMA)检测肾综合征出血热(HFRS)患者血清抗HFRS病毒特异性抗体IgM和IgG及细胞因子含量的新方法。方法:选择28例临床确诊的HFRS患者及20例健康人血清标本,FMA定量检测抗HFRS病毒IgM和IgG;定量检测细胞因子IL-6和TNF-α。检测结果与ELISA法进行比较。结果:FMA检测HFRS患者抗HFRS病毒IgM和IgG的阳性率分别为92.85%和71.43%,健康对照组的抗体阳性率(假阳性率)为0;HFRS患者血清IL-6和TNF-α的含量分别为(532.62±397.19)ng/L和(392.68±177.68)ng/L,明显高于健康对照组(38.77±20.32)ng/L(P<0.01)和(15.91±6.91)ng/L(P<0.01)。ELISA法检测HFRS患者抗HFRS病毒IgM和IgG的阳性率分别为71.43%和50.00%,健康对照组的抗体阳性率(假阳性率)为0;HFRS患者血清IL-6和TNF-α的含量分别为(256.46±102.51)ng/L和(45.63±5.32)ng/L,高于健康对照组(53.8±19.21)ng/L(P<0.01)和(5.81±3.58)ng/L(P<0.01)。结论:建立了FMA法对HFRS患者的特异性抗体和IL-6和TNF-α的检测,其灵敏度明显优于ELISA法,为HFRS的临床诊断和病理机制研究提供了新的方法。
OBJECTIVE: To establish a new method for detecting serum anti-HFRS virus-specific IgM and IgG and cytokines by flow cytometry (FMA) in patients with hemorrhagic fever with renal syndrome (HFRS). Methods: Serum samples were collected from 28 clinically diagnosed HFRS patients and 20 healthy volunteers. FMA was used to detect anti-HFRSV IgM and IgG. The cytokines IL-6 and TNF-α were detected quantitatively. The test results were compared with ELISA. Results: The positive rates of anti-HFRS virus IgM and IgG in patients with HFRS by FMA were 92.85% and 71.43%, respectively. The antibody positive rate (false positive rate) in healthy controls was 0. The levels of IL-6 and TNF- (532.62 ± 397.19) ng / L and (392.68 ± 177.68) ng / L, respectively, which were significantly higher than those in healthy controls (38.77 ± 20.32 ng / L, P <0.01 and 15.91 ± 6.91 ng / L, respectively <0.01). The positive rates of anti-HFRS virus IgM and IgG in HFRS patients were 71.43% and 50.00%, respectively, while the antibody positive rate (false positive rate) in healthy controls was 0. The contents of IL-6 and TNF- (P <0.01) and (5.81 ± 3.58) ng / L, respectively (P <0.01) in the control group (256.46 ± 102.51 ng / L and (45.63 ± 5.32) ng / L, compared with 53.8 ± 19.21 ng / ). Conclusion: The FMA method was used to detect the specific antibodies and IL-6 and TNF-α in patients with HFRS. The sensitivity was better than ELISA, which provided a new method for the clinical diagnosis and pathological study of HFRS.