论文部分内容阅读
目的：探究金纳米棒引发癌细胞凋亡的机制。方法利用金种子生长法合成金纳米棒，并对其进行表征；利用CCK-8评价金纳米棒对细胞的增殖抑制-毒性，并通过流式凋亡检测；利用 JC-1对线粒体膜电位检测及 western blot 检测Caspase9和Caspase3的活化程度。结果通过研究发现金纳米棒对正常细胞毒性很小，而对肿瘤细胞毒性很大；并且随着金纳米棒作用时间增加癌细胞的凋亡比例也在增加；另外正常细胞线粒体膜电位没变化而癌细胞线粒体膜电位大幅度降低。结论金纳米棒引发的癌细胞凋亡是通过线粒体凋亡途径。“,”Objective To explore the mechanism that gold nanorods trigger apoptosis in cancer cells.Methods Gold nanorods was synthesized by gold seed growing method, and its characterization was detected; gold nanorods on cell proliferation-toxicity were evaluated by CCK-8 Kit and apoptosis were detected by flow; mitochondrial membrane potential were tested by JC-1 and activation of Caspase 9 and Caspase 3 were detected by western blot. Results The results found that gold nanorods had nontoxic to normal cells, but highly toxic to tumor cells; and with the increasing of gold nanorods’ working time, the percentage of apoptotic cancer cells was increasing; in addition to, normal cells’ mitochondrial membrane potential did not change, but cancer cells had a significant reduction in mitochondrial membrane potential.Conclusion This study proves that gold nanorods induce apoptosis through the mitochondrial apoptosis pathway.