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目的:建立颈n 7根性撕脱诱发神经病理性痛的小鼠模型，并验证其可靠性。n 方法:选用雌性C57小鼠36只，随机分为假手术组、撕脱伤组。撕脱伤组于前路行颈n 7根性撕脱术；假手术组仅暴露并分离颈n 7神经根。术前和术后，检测小鼠患侧前足痛行为、双侧前足肌力及Rota-rod实验。术后7 d，取颈n 7节段脊髓行免疫荧光染色，并进行统计学分析。n 结果:与假手术组相比，撕脱伤组出现明显机械痛敏和冷痛敏(n P0.05)。免疫荧光染色结果显示术后7 d，撕脱伤组脊髓背角GFAP及Iba1表达较假手术组显著升高(n P<0.05)。n 结论:此单纯颈n 7根性撕脱小鼠模型可显著诱发神经病理性痛，且对患肢运动功能无明显损伤，可作为研究臂丛神经撕脱伤诱发神经病理性痛的理想动物模型。n “,”Objective:To establish a mouse model of neuropathic pain induced by Cn 7 root avulsion and verify its reliability.n Methods:Thirty-six female C57 mice were randomly divided into sham operation group and brachial plexus avulsion group. In the avulsion group, the Cn 7 root was avulsed through anterior approach; in the sham operation group, Cn 7 root was exposed and separated. Before and after the operation, the pain behavior of the affected side of the forepaw, the muscle strength of the bilateral forepaws and the Rota-rod test were measured. At the 7th day after the operation, the segment of Cn 7 spinal cord was havested to stain with immunofluorescence, and the data were analyzed statistically.n Results:Compared with the sham operation group, the avulsion group had significant mechanical and cold pain hypersensitivity (n P0.05). The immunofluorescence staining results showed that the expression of GFAP and Iba1 in spinal dorsal horn of avulsion group was significantly higher than that of sham operation group 7 days after operation (n P<0.05).n Conclusion:This simple Cn 7 nerve avulsion mouse model can induce neuropathic pain and has no significant damage to the motor function of the affected limb. It can be used as an ideal animal model to study neuropathic pain induced by brachial plexus avulsion.n