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目的:探讨三七总皂苷对脊髓损伤后的保护作用以及对GFAP表达变化的影响。方法:健康成年雌性SD大鼠63只,随机分为正常组,溶媒对照组,三七总皂苷组,大鼠脊髓T10右侧半横断损伤后15min,腹腔注射三七总皂苷,剂量为20mg.kg-1,以后每天给药一次,溶媒对照组注射等量生理盐水。术后1d,3d,7d,14d,21d,28d进行BBB评分行为学检测;动物存活1d、3d、7d、14d、28d,运用免疫组织化学方法检测脊髓损伤远侧端GFAP表达的变化。结果:BBB评分显示,三七总皂苷能明显促进脊髓损伤后运动功能的恢复,其中损伤后7d和14d的评分明显高于溶媒对照组。免疫组化结果显示脊髓T10右侧半横断损伤后,脊髓远侧段GFAP的表达损伤侧均强于对侧,损伤侧灰质GFAP的表达呈现出1d,3d逐渐增强,7d达高峰的趋势,14dGFAP的表达逐渐下降,至28d仍略高于正常组。三七总皂苷组和溶媒对照组相比,GFAP表达的时间趋势相同,但相同时间点GFAP的表达弱于对照组,尤其是3d、7d。结论:三七总皂苷能抑制脊髓半横断损伤后星形胶质细胞的活化,这可能是其促进脊髓损伤后运动功能恢复的机制之一。
Objective: To investigate the protective effect of Panax notoginseng saponins on spinal cord injury and its effect on the expression of GFAP. Methods: Sixty-three healthy adult female Sprague-Dawley rats were randomly divided into normal group, vehicle control group, total saponins of Panax notoginseng group and 15 min of right half-transected T10 of spinal cord in rats. kg-1, once daily after administration, vehicle control group injected with the same amount of saline. BBB score behavior was detected at 1d, 3d, 7d, 14d, 21d and 28d after operation. The animals survived for 1d, 3d, 7d, 14d and 28d. The expression of GFAP in distal spinal cord was detected by immunohistochemistry. Results: BBB score showed that Panax Notoginseng Saponins could obviously promote the recovery of motor function after spinal cord injury, and the scores at 7d and 14d after injury were significantly higher than that of vehicle control group. The results of immunohistochemistry showed that the expression of GFAP in the distal segment of the spinal cord was stronger on the injured side than that on the contralateral side after injury of the right hemisected T10. The expression of GFAP on the injured side showed a trend of increasing on the 1st and 3rd day and reaching the peak on the 7th day. The expression gradually decreased to 28d still slightly higher than the normal group. Compared with the vehicle control group, the time trend of GFAP expression was the same, but the expression of GFAP at the same time point was weaker than that of the control group, especially at 3d and 7d. Conclusion: Panax notoginseng saponins can inhibit the activation of astrocytes after spinal cord transection, which may be one of the mechanisms that promote the recovery of motor function after spinal cord injury.