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To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), a novel factor cloned in our laboratory recently, in erylhroid terminal differentiation.Methods. Mouse erythroleukemia (MEL) cells were transfected with eukaryotie expression plasmid pcD-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and β-globin genes were analysed by semi-quantitative RT-PCR.Results. MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index,and colony-forming rate in semi-solid medium ( P<0. 01). The percentage of benzidine-positive cells was 32. 8%after transfection. The expression of β-globin in cells transfected with pcDNA-MEDDF was 3. 43 times higherthan that of control (MEL transfected with blank vector, pcDNA3. 1), and the expression of c-mye decreased by66. 3% .Conclusions. MEDDF can induce differentiation of
To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), a novel factor cloned in our laboratory recently, in erylhroid terminal differentiation. Methods. Mouse erythroleukemia (MEL) cells were transfected with eukaryotie expression plasmid pcD-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and β-globin genes were analysed by semi-quantitative RT-PCR. Results . MEL cells transfected with significant lower growth rate, mitotic index, and colony-forming rate in semi-solid medium (P <0.01). The percentage of benzidine-positive cells was 32. 8% after transfection. The expression of β-globin in cells transfected with pcDNA-MEDDF was 3. 43 times higherthan that of control (MEL transfected with blank vector, pcDNA3. 1), and the expression of c-mye decreased by 66.3% .Conclusions. MEDDF can induce differentiation of