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目的在活体分子影像学研究中寻找适当的门静脉入路,并阐述门静脉影像解剖特征。材料和方法成年SD大鼠30只按穿刺部位的不同分为脾静脉组、门静脉主干组和盲肠静脉组各10只。苯巴比妥麻醉下剖腹解剖大鼠,以0.4号静脉针穿刺脾静脉、门静脉主干、盲肠静脉,造影并测量门静脉及附属血管的相关数据,观测3处穿刺点的解剖特征、穿刺成功率、大鼠死亡情况、实验成功率,以此来选择合适的穿刺点。结果脾静脉、门静脉主干、盲肠静脉的穿刺成功率分别为30%,70%和100%,实验成功率分别为20%、40%和90%。手术创伤、失血是大鼠死亡的主要原因。盲肠静脉穿刺成功率及实验成功率明显高于脾静脉组和门静脉主干组。结论经大鼠盲肠静脉穿刺简便易行,是进行门静脉系统相关分子影像学研究的合适入路。
Objective To search for appropriate portal vein approach in living molecular imaging and to elucidate the anatomic features of portal vein. Materials and Methods Thirty adult Sprague-Dawley rats were divided into spleen vein group, portal vein trunk group and cecum vein group respectively according to different puncture sites. The rats were dissected by phenobarbital anesthesia, the splenic vein, portal vein, cecum vein and angiography were performed with 0.4 needle, and the portal vein and accessory blood vessels were measured. The anatomic characteristics of the three puncture points, the successful rate of puncture, Rat death, experimental success rate, in order to select the appropriate puncture point. Results The success rates of splenic vein, portal vein and cecum vein were 30%, 70% and 100%, respectively. The successful rates were 20%, 40% and 90% respectively. Surgical trauma, blood loss is the main cause of death in rats. The success rate and successful rate of cecum venous puncture were significantly higher than those of splenic vein and portal vein. Conclusions The caecum venovenous puncture in rats is simple and convenient, which is a suitable approach for molecular imaging studies related to the portal system.