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Homeobox transcription factors participate in the growth and development of plants by regulating cell differentiation, morphogenesis and environmental signal response. To reveal the functions of these transcription factors in rice, we constructed the RNAi vectors of Os Hox9, a member of homeobox family, and analyzed the function of Os Hox9 using reverse genetics. The plant height and tillering number of RNAi transgenic plants decreased compared with those of wild-type plants. Reverse transcription-polymerase chain reaction analysis showed that Os Hox9 expression reduced in the transgenic plants with phenotypic variance, whereas that in the transgenic plants without phenotypic variance was similar to that in the wild-type plants. This result suggests that the phenotypes of the transgenic plants were caused by RNAi effects. The tissue-specificity of Os Hox9 expression indicated that it was expressed in different organs, with high expression in stem apical meristem and young panicles. Subcellular location of Os Hox9 demonstrated that it was localized on the cell membrane.
Homeobox transcription factors participate in the growth and development of plants by regulating cell differentiation, morphogenesis and environmental signal response. To reveal the functions of these transcription factors in rice, we constructed the RNAi vectors of Os Hox9, a member of homeobox family, and analyzed the function of Os Hox9 using reverse genetics. The plant height and tillering number of RNAi transgenic plants decreased compared with those of wild-type plants. Reverse transcription-polymerase chain reaction analysis showed that Os Hox9 expression reduced in the transgenic plants with phenotypic variance, yet that in the transgenic plants without phenotypic variance was similar to that in the wild-type plants. This result suggests that the phenotypes of the transgenic plants were caused by RNAi effects. The tissue-specificity of Os Hox9 expression indicates that it was expressed in different organs, with high expression in stem apical meristem and young panicles. Sub cellular location of Os Hox9 demonstrated that it was localized on the cell membrane.