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采用蛋白芯片竞争法对小分子半抗原的污染物进行检测。在获得特异性抗体的前提下,首先将阿特拉津半抗原进行了衍生化,然后将该衍生物和氨基罂粟碱分别与载体蛋白质卵清蛋白(OVA)进行偶联。实验证明新合成的完全抗原能够与其相应抗体发生特异性的结合。实验还对蛋白芯片检测阿特拉津进行了条件优化,其抗体固定化时间为2h,用卵清蛋白为封闭液的封闭时间为1h,样品稀释液pH值为8. 0。并对阿特拉津及罂粟碱进行了定性、定量实验,结果表明:荧光信号强度随待测物浓度的降低而增强,有一定的线性趋势,阿特拉津检出限为0. 001mg/L,罂粟碱检出限为0. 01mg/L。
Protein chip competition was used to detect small molecule hapten contaminants. In order to get the specific antibody, we first derivatized the atrazine titera antigen, and then conjugated the derivative and amino-papaverine to the carrier protein ovalbumin (OVA). Experiments show that the newly synthesized complete antigen can bind specifically with its corresponding antibody. The experiment also optimized the protein chip detection of atrazine, the antibody immobilization time was 2h, the incubation time with ovalbumin as a blocking solution was 1h, the pH value of the sample diluent was 8.0. And atrazine and papaverine were qualitative and quantitative experiments, the results showed that: fluorescence signal intensity decreases with the concentration of the test substance increased, there is a certain linear trend, atrazine detection limit of 0. 001mg / L, papaverine detection limit of 0. 01mg / L.