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目的了解引起江苏省2005~2006年麻疹流行的麻疹野病毒基因特征,为制定消除麻疹策略提供依据。方法2005~2006年收集了324例来自江苏省7个设区市的麻疹急性期病人咽拭子标本,用EB病毒转化的狨猴淋巴母细胞(B95a细胞)从5个市分离到麻疹病毒99株。用逆转录-聚合酶链反应从99株麻疹病毒中扩增出核蛋白(N)基因3′端676个核苷酸片段,并对该片段进行序列测定和分析,构建基因亲缘关系树。结果通过N基因3′端450个核苷酸片段的序列测定和分析证明,99株均为麻疹病毒H1基因型中的H1a基因亚型,H1a基因亚型中有2个小分支。99株麻疹病毒间450个核苷酸差异率为0%~4.7%;与A基因型代表株Edmonston株的核苷酸差异率为6.7%~9.8%;与H2基因型代表株China94-1的核苷酸差异率为6.7%~9.6%。结论H1a基因亚型麻疹病毒在江苏省广泛流行,为绝对优势基因亚型,H1a亚型2个小分支中的很多不同或相同的病毒株引起的多个传播链造成省内各市的麻疹广泛传播。
Objective To understand the genetic characteristics of wild measles virus that caused the measles epidemic in Jiangsu Province from 2005 to 2006, and to provide evidence for the development of measles elimination strategy. Methods A total of 324 throat swabs were collected from 2005-2006 in acute measles patients from 7 districts in Jiangsu Province. Measles virus 99 was isolated from 5 cities using EB virus-transformed marmoset lymphoblasts (B95a cells) Strain. The 676 nucleotides of the 3 ’end of the nucleoprotein (N) gene were amplified from 99 measles viruses by reverse transcription - polymerase chain reaction (PCR) and sequenced and analyzed. The gene phylogenetic tree was constructed. Results The sequencing and analysis of the 450 nucleotide sequences of the 3 ’end of N gene showed that all of the 99 isolates were H1a subtypes of the H1 genotype and 2 minor subtypes of the H1a subgenotype. The difference of 450 nucleotides between 99 strains of measles virus was 0% -4.7%. The nucleotide variation rate of Edmonston strain with A genotype was 6.7% -9.8% The nucleotide difference rate was 6.7% ~ 9.6%. Conclusion H1a subtype measles virus is widely prevalent in Jiangsu Province and is an absolute predominant gene subtype. Multiple transmission lines caused by many different or same virus strains in two subtypes of H1a subtype caused wide spread of measles in different cities in the province .