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本研究对某厂１２２名接触１─〔２．４─二硝基〕苯氧基—５—〔３．４—二硝基〕苯氧基—４．８—二硝基蒽醌（ＤＤＮＡＱ）（车间空气粉尘浓度为０．１６ｍｇ／ｍ３）的工人和某厂１００名非接触工人进行了口腔粘膜上皮细胞微核检查，发现接触组微核人员出现率和平均微核率分别为７６．２２％和２％，显著高于非接触组相应的３０．００％（Ｐ＜０．０１）和０．４％（Ｐ＜０．０１）。是时，上述接触组和非接触组各１２名工人进行了中性白细胞ＤＮＡ含量测定，接触组（６００个细胞）ＤＮＡ含量为３．６７±０．３０，与非接触组（６００个细胞）的３．０２±０．１８相比较，差别显著（Ｐ＜０．０１）。此外，动物实验结果表明，小鼠受精卵染色体数目畸变细胞率，染毒组（０．８ｍｇ／ｋｇ·ｗｔ）为１８．７５％，显著高于对照组的８．７４％（Ｐ＜０．０５）；金黄地鼠胚胎早期培养细胞转化试验，染毒组（０．１ｕｇ／ｍｌ，１．０ｕｇ／ｍｌ转化克隆／总克隆为４／４６７、３／４４２（空白对照组为０／５０８），呈阳性结果。上述结果说明ＤＤＮＡＱ对遗传物质存在作用，应加强职业性接触工人卫生防护工作，保护工人及其后代的健康。
In this study, 122 plants exposed to 1 - [2,4-dinitro] phenoxy-5- [3.4-dinitro] phenoxy-4.8-dinitroanthraquinone (DDNAQ) (Workshop dust concentration of 0.16mg / m3) workers and a factory 100 non-contact workers oral mucosal epithelial cell micronucleus examination found that the contact group micronucleus frequency and the average micronucleus rate were 76.22 % And 2%, respectively, were significantly higher than those of the non-contact group (30.00% and 0.4%, P <0.01). The DNA content of the contact group (600 cells) was 3.67 ± 0.30, compared with the non-contact group (600 cells) in the 12 contact and non-contact groups, respectively. 3.02 ± 0.18, the difference was significant (P <0.01). In addition, animal experiments showed that the number of chromosome aberration cells in fertilized eggs of mice was significantly higher than that of the control group (18.75%, 0.8 mg / kg · wt) (8.74%, P <0. 05). In the early stage of golden hamster embryo culture, the transformation of cells in embryo culture was observed. In the exposure group (0.1ug / ml, 1.0ug / ml transformed clone / total clone 4 / 467,3 / 442 (0/508 in blank control group) , Positive results.The above results show that DDNAQ on genetic material role should be strengthened occupational exposure workers in the work of health protection to protect the health of workers and their offspring.