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目的 通过观察缺氧诱导因子2α(HIF -2α)在妊娠高血压综合征和正常妊娠胎盘组织中表达的差异,探讨HIF -2α与妊娠高血压综合征发病过程的关系,为妊娠高血压综合征的诊治提供新的理论依据。方法 收集10例妊娠高血压综合征和10名年龄配对的正常妊娠胎盘组织,运用蛋白质印迹法(westernblot) ,检测HIF 2α蛋白的表达水平;应用实时聚合酶链反应(real timePCR)方法,检测HIF -2αmRNA的表达水平。结果 与正常妊娠胎盘组织相比,妊娠高血压综合征胎盘中HIF -2α蛋白和mRNA表达水平均增高。结论 HIF -2α在妊娠高血压综合征胎盘组织中的异常高表达,进一步证明缺氧在妊娠高血压综合征发病中的作用,其高表达可能与妊娠高血压综合征的病因及病理过程有关。
OBJECTIVE: To investigate the relationship between HIF-2α and the pathogenesis of pregnancy-induced hypertension (Hypertension syndrome) by observing the difference of HIF-2α expression in pregnancy-induced hypertension syndrome and normal pregnancy placenta, Provide new theoretical basis for diagnosis and treatment. Methods Ten cases of pregnancy-induced hypertension and 10 normal-matched placental tissues were collected. Western blotting was used to detect the expression of HIF-2α protein. HIF-1α was detected by real-time polymerase chain reaction (real time PCR) -2α mRNA expression level. Results Compared with normal pregnancy placenta, the expression of HIF-2α protein and mRNA in placenta of pregnancy-induced hypertension increased. Conclusions HIF - 2α is abnormally expressed in placenta of pregnancy induced hypertension, further demonstrating the role of hypoxia in the pathogenesis of pregnancy - induced hypertension. The high expression of HIF - 2α may be related to the etiology and pathological process of pregnancy - induced hypertension.