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为研究压力对筛板细胞形态和糖蛋白合成的影响，对筛板细胞进行体外培养并施与一定压力，相差显微镜下观察筛板细胞形态学改变，并采用显微荧光光度计对筛板细胞合成层粘蛋白（ＬａｍｉｎａＬＮ）及纤维连接蛋白（ｆｉｂｒｉｎｅｃｔｉｏｎＦＮ）进行定量测定。结果在静压力（５０ｍｍＨｇ）下，筛板细胞形态变扁，随加压时间延长，胞浆内空泡增多，线粒体肿胀、部分空泡化；胞浆内可见髓鞘样结构；加压３天的筛板细胞合成ＬＮ及ＦＮ分别为４２．２±４．６７、６３．６５±１１．２０荧光强度（ＡＵ），与对照组比较，差异呈显著性（Ｐ＜０．０５），加压５天的筛板细胞合成ＬＮ及ＦＮ分别为３６．６５±８．４５、６０．９０±１１．９１ＡＵ，与对照组比较仍有不同程度增高。显示压力能促使筛板细胞形态异常和糖蛋白合成增加。
In order to study the effect of pressure on cell morphology and glycoprotein synthesis, the cells were cultured in vitro and subjected to a certain pressure. The morphological changes of the cells were observed under a phase contrast microscope. LaminaLN and fibrinectionFN were measured quantitatively. Results Under the static pressure (50mmHg), the morphology of the sieve plate became flat. With the prolongation of the pressure time, the cytoplasm vacuolus increased and the mitochondria became swollen and partially vacuolized. The myelin-like structure was observed in the cytoplasm. After 3 days (P <0.05). Compared with the control group, the LN and FN of the platelet cells were 42.2 ± 4.67 and 63.65 ± 11.20, respectively. The fluorescence intensity (AU) The synthesis of LN and FN in the 5-day platelet cells were 36.65 ± 8.45 and 60.90 ± 11.91 AU respectively, which were still increased to some extent compared with the control group. Show pressure can promote abnormal cell morphology and glycoprotein synthesis.