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目的 探讨神经酰胺在表没食子儿茶素没食子酸酯 (EGCG)、表没食子儿茶素 (EGC)和表儿茶素 (EC)诱导大肠癌LoVo细胞凋亡及其差异性中的作用。方法 EGCG ,EGC和EC以及C2 和C6 神经酰胺处理LoVo细胞后 ,用琼脂糖凝胶电泳 ,Heochst染色、流式细胞术定性和定量检测LoVo细胞凋亡相关的形态学和生化方面的改变 ;用二酰基甘油激酶法测定 3种儿茶素处理LoVo细胞后其神经酰胺含量的变化。结果 EGCG和EGC处理LoVo细胞后 ,可引起LoVo细胞的凋亡 ,C2 和C6 神经酰胺也可引起相似的改变 ,但EC则无此作用。EGCG或EGC处理LoVo细胞后细胞内神经酰胺含量先一短暂的升高后逐渐回到基线水平 ,而EC则先引起短暂的下降再逐渐回升至基线水平。结论 3种儿茶素在诱导LoVo细胞凋亡时呈现出差异性 ,这种差异性与它们引起LoVo细胞内神经酰胺含量变化的差异性有关
Objective To investigate the role of ceramide in the apoptosis and the difference of colorectal cancer LoVo cells induced by epigallocatechin gallate (EGCG), epigallocatechin (EGC) and epicatechin (EC). Methods LoVo cells were treated with EGCG, EGC, EC and C2 and C6 ceramides. The morphological and biochemical changes of LoVo cells were qualitatively and quantitatively detected by agarose gel electrophoresis, Heochst staining and flow cytometry. Changes of ceramide content in LoVo cells treated with 3 kinds of catechins by diacylglycerol kinase method. Results After LoCo cells were treated with EGCG and EGC, apoptosis of LoVo cells was induced. C2 and C6 ceramide also induced similar changes, but EC did not. The intracellular ceramide content of EGCG or EGC treated with LoVo cells gradually increased to a certain level and then returned to baseline level. However, EC decreased first and then gradually returned to the baseline level. Conclusion The three kinds of catechins showed differences in the induction of apoptosis in LoVo cells, which was related to the difference in the content of ceramide in LoVo cells