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用植物源抗病毒制剂VA与烟草花叶病毒 (TMV)混合 ,经枯斑半叶法接种 ,结果证明 ,VA对TMV有较强的体外钝化作用 ,体外钝化后的枯斑抑制率为 80 .0 % ;用苯酚氯仿法提取TMV的核酸后 ,按一定的比例与VA混合 ,经枯斑半叶法接种测得枯斑抑制率为 43.8%～ 77.9% ;TMV与VA混合后用RNase处理 ,测得侵染率为 78.6 % ,比未用RNase处理的对照降低了 2 1.4% ,由此说明VA具有一定的体外脱衣壳作用 ;又经电镜观察发现VA可使部分TMV粒体发生断裂或粗细不均、略变弯曲等畸变现象。
VA was mixed with Tobacco Mosaic Virus (TMV) and inoculated by semi-leaf blight. The results showed that VA had a strong in vitro passivation effect on TMV, and the inhibition rate of in vitro passivation was 80.0%. The nucleic acid of TMV was extracted by phenol-chloroform method and then mixed with VA at a certain ratio. The inhibition rate of dead spot was 43.8% -77.9% after half-leaf seeding inoculation. TMV and VA were mixed with RNase Treatment, the measured infection rate was 78.6%, compared with the control without RNase treatment decreased by 21.4%, indicating that VA has some in vitro decapsulation effect; and observed by electron microscopy found that some of the TMV particles can be broken Or uneven thickness, slight distortion and other phenomena.