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目的研究经大鼠溃疡皮肤反复给予朱红膏对肾组织丙二醛(MDA)、Na+-K+-ATP酶及金属硫蛋白(MT)的影响,初步探讨朱红膏中汞经皮吸收作用于肾的机理。方法将80只SD大鼠随机分为8组:朱红膏A组(1218.56 mg/kg)、B组(609.28 mg/kg)、C组(304.64 mg/kg)、D组(152.32 mg/kg)、E组(76.16 mg/kg)5个剂量组及基质组(凡士林)、溃疡模型组、皮肤破损组。溃疡模型采用皮肤缺损+埋置异物+细菌感染法造模,皮肤破损组仅以刀片刮伤大鼠皮肤,基质组及朱红膏各剂量组将药纱条直接贴敷于创面,每日给药4 h,连续给药14 d。测定肾组织中MDA含量、Na+-K+-ATP酶活力,并采用酶联免疫分析法测定MT含量。结果与溃疡模型组比较,朱红膏A组MDA含量升高(P<0.05);朱红膏A组Na+-K+-ATP酶活力显著降低(P<0.01),基质组与朱红膏C、D、E组酶活力均呈不同程度的升高;朱红膏A、B、C、D组MT含量均显著升高(P<0.01),其余差异无统计学意义(P>0.05)。结论反复过量使用朱红膏,汞经皮吸收作用于肾脏,可以引起脂质过氧化,影响能量代谢,诱导MT的产生。
Objective To investigate the effect of repeated administration of Zhuhong cream on the renal tissue malondialdehyde (MDA), Na + -K + -ATPase and metallothionein (MT) in rat ulcer skin, and to investigate the effect of percutaneous absorption of mercury on the renal mechanism. Methods Eighty SD rats were randomly divided into 8 groups: control group A (1218.56 mg / kg), group B (609.28 mg / kg), group C (304.64 mg / kg), group D (152.32 mg / kg) , E group (76.16 mg / kg), five dose groups and matrix group (petrolatum), ulcer model group and skin lesion group. The ulcer model was made by skin defect + embedded foreign body + bacterial infection method. The skin damage group only scratched the rat’s skin with a blade, and the matrix and the red cream each dose group applied the gauze directly to the wound, 4 h, continuous administration 14 d. The content of MDA and the activity of Na + -K + -ATP in renal tissue were measured, and the content of MT was determined by enzyme-linked immunosorbent assay. Results Compared with the ulcer model group, the content of MDA in Zhuhong cream group A increased (P <0.05); the activity of Na + -K + -ATPase in Zhuhong cream group A decreased significantly (P <0.01); the matrix group and Zhuhong paste C, D, E The activity of MTT in group A, B, C, D increased significantly (P <0.01), but the difference was not statistically significant (P> 0.05). Conclusion Repeated excessive use of Zhu Hong paste, mercury percutaneous absorption of the role in the kidneys, can cause lipid peroxidation, affecting energy metabolism, induction of MT.