Cloning of Bt cry Genes by Rapid Screening of DNA Libraries with PCR—RFLP

• 作 者: CHEN－Zhong－yi HUANGDa－fang 等
• 机 构: StateKeyLaborataryforBiologyofPlantDiseasesandInsectPests,BiotechnologyResearchInstitute
• 出 处: 中国农业科学：英文版
• 发表日期: 2003年2期
• 关键词: 苏云金牙胞杆菌Cry基因 杀虫蛋白 DNA文库 PCR-RFLP 快速筛检 基因克隆
• 基金项目: the National Transgenic Project of China

Bacillus thuringiensis (Bt) strain C002 contains crylAa, cry2Ab, cry1Ca insecticidal crystal genes and an unkown gene cryX, among which crylCa is located in a 6 -9 kb EcoR Ⅰ fragment of the chromosomal DNA. The total DNA and the plasmids DNA libraries of