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目的:观察角膜中央碱性烧伤后角膜上皮的形态学变化.方法:用浸有0.5mol/L NaOH溶液的8mm直经圆形纸片放在39只兔角膜中央表面1分钟,制备兔角膜中央碱性烧伤模型,用荧光素染色裂隙灯检查、光镜、扫描电镜和透射电镜进行研究.结果:角膜烧伤后即出现上皮溶解脱落,伤后8小时烧伤边缘处上皮细胞呈梭形,向损伤区内伸出突起;24小时大部分区域已有单层上皮覆盖,第4天角膜上皮再脱落.修复的上皮表面微绒毛和桥粒结构少,细胞间隙明显增宽.结论:角膜中央碱性烧伤上皮愈合主要是通过自外周正常区上皮细胞向损伤区增生移行来完成.再生的角膜上皮细胞间连接结构的减少可能与上皮易脱落有关.眼科学报1999;15:74?77.“,”Purpose:To observe the morphologic changes of epithelium in the alkali wounds at the central corneal of rabbits.Methods :38 rabbits weighing 2. 5kg were used. A alkali wound of the corneas was performed in the right eye of 38 New Zealand white rabbits with round filter paper, 8mm in diameter, which were soaked in 0. 5mol/L NaOH for 5 seconds and then were placed centrally on the cornea for 60 seconds. The corneal burns were examined and photographed after the staining of 2% fluoresent staining with a slit lamp every day. The excised corneas were examined in the light microscope, electron microscope.Results :The epithelial cells had rapidly disappeared in the burned area. The epithelial cells regrowth started at 8 hours following the burn, and 1-2 layers epithelial cells covered the burned area by 24 hours. The epithelial defects occured again on the 4th day. The desmosome and nnicrovilli of the regenerating epithelium had a reduced number. There was no basement membrane between the regenerating epithelium and the strom. There were increased intercellular spaces between the regenerating epi-thalial cells.Conclusion ;The epithelial healing were achieved through the division and migration of epithelial cells at the margin of the wound in rabbit central corneal alkali wound. The decrease of the junctions and the increase of intercellular spaces between the regenerating epithalial cells may be responsible for the second epithelial defects. Eye Science 1999; 15: 74 - 77.