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1.细菌污染提纯法: (1)材料:含量为4万单位/1毫升的庆大霉素一支;经高压灭菌的PDA斜面培养基10支(试管规格18×180毫米);高压灭菌的1毫升注射器一支;6号针头一个。 (2)方法:在无菌箱(室)内,把4万单位/1毫升的庆大霉素液用砂轮割开,用无菌注射器分别注入10支冷却至45℃左右的斜面试管培养基中,摇动培养基使之充分混匀,立即摆成斜面。然后把被细菌污染的菌种以无菌操作移入该斜面培养基中,置25℃下培养。2次即可把细菌甩掉,效果甚佳,对食用菌菌丝无抑制作用。 2.霉菌污染提纯法: (1)试管棉塞受潮污染了霉菌,大都出现在斜面前端,可在酒精灯火焰上连同培养基一块灼
1. Bacterial pollution purification method: (1) Material: a content of 40,000 units / 1 ml of gentamicin; autoclaved PDA slant medium 10 (test tube size 18 × 180 mm); high pressure off A 1 ml syringe for bacteria; a 6-gauge needle. (2) Method: In aseptic chamber (chamber), 40,000 units / 1 ml of gentamicin solution with a grinding wheel cut off, with a sterile syringe were injected into the 10 cooled to 45 ° C bevel test tube medium Medium, shake the medium to make it fully mixed, immediately put into a bevel. The bacteria-contaminated species are then aseptically transferred into the slant culture medium and incubated at 25 ° C. 2 times to get rid of bacteria, the effect is very good, no inhibition of edible fungi mycelium. (2) mildew pollution purification method: (1) tube tampon damp mildew contaminated, mostly appear in front of the bevel, in the alcohol lamp flame with a burning medium