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目的探讨β-七叶皂苷钠对大鼠脑出血后TNF-α及脑水肿的影响。方法采用Rosenberg脑内立体定向注射Ⅶ型胶原酶诱发大鼠脑出血模型。将SD大鼠78只随机分为假手术组、出血组、出血干预组及对照组。以干湿重法测定脑含水量,用ELASA法测量血清TNF-α含量。利用草酸-焦锑酸钾电镜化学技术观察脑出血周围组织超微结构的改变。结果治疗组脑含水量明显低于对照组(P<0.01)。出血后12h TNF-α升高达高峰,此后逐渐下降,β-七叶皂苷钠治疗组在出血后24h及3d较出血组及对照组明显减少,7d则与后两组无明显差异。超微结构观察治疗组神经元及内皮细胞肿胀程度较对照组明显减轻。结论β-七叶皂苷钠能明显减少脑出血模型大鼠血清TNF-α水平以及减轻脑水肿。
Objective To investigate the effect of sodium β-aescin on TNF-α and brain edema after intracerebral hemorrhage in rats. Methods The intracerebral hemorrhage model induced by intratumoral injection of type VII collagenase was induced by Rosenberg. 78 SD rats were randomly divided into sham operation group, bleeding group, hemorrhage intervention group and control group. Brain water content was measured by dry-wet weight method and serum TNF-α content was measured by ELASA method. The ultrastructural changes of the tissues around the intracerebral hemorrhage were observed using the oxalic acid-potassium pyroantryate electron microscope technique. Results The brain water content in the treatment group was significantly lower than that in the control group (P<0.01). The peak of TNF-α increased at 12h after hemorrhage and then decreased gradually. The β-sodium aescinate treatment group decreased significantly at 24h and 3d after hemorrhage compared with the bleeding group and the control group, and there was no significant difference between 7d and 7d groups. The ultrastructural observation of the swelling of neurons and endothelial cells in the treatment group was significantly less than that in the control group. Conclusions β-aescin can significantly reduce serum TNF-α levels and reduce cerebral edema in rats with cerebral hemorrhage.