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目的:观察腹腔注射全氟化碳(PFC)对心肌缺血大鼠单核细胞趋化蛋白-1(MCP-1)水平的影响,初步探讨PFC对大鼠缺血心肌的可能保护作用。方法:将50只Wistar雄鼠随机分为5组(每组10只):假手术组、缺血组、低剂量组、中剂量组、高剂量组。采用结扎冠状动脉前降支1h造成大鼠心肌缺血损伤模型。用ELISA方法检测缺血20min、40min及60min时外周血MCP-1水平,并观察缺血心肌的病理变化。结果:各组外周血MCP-1水平随着缺血时间延长呈逐渐升高趋势,缺血组与假手术组比较外周血MCP-1水平明显增加(P<0.05);低剂量组与缺血组相比MCP-1水平差异无统计学意义(P>0.05),缺血40min、60min时,中、高剂量组与缺血组相比MCP-1水平显著减少(P<0.05),但在缺血20min时中剂量组与缺血组相比差异无统计学意义(P>0.05),高剂量组与中剂量组相比,MCP-1水平降的更低(P<0.05);中高剂量PFC可减轻缺血心肌的组织形态学改变。结论:腹腔注射PFC能够降低心肌缺血大鼠外周血MCP-1水平,对缺血心肌起到一定的保护作用。
OBJECTIVE: To observe the effect of intraperitoneal injection of perfluorocarbon (PFC) on monocyte chemoattractant protein-1 (MCP-1) in myocardial ischemic rats and to explore the possible protective effect of PFC on ischemic myocardium in rats. Methods: Fifty Wistar male rats were randomly divided into 5 groups (10 in each group): sham operation group, ischemia group, low dose group, middle dose group and high dose group. Ligation of anterior descending coronary artery 1h myocardial ischemia injury model in rats. The levels of MCP-1 in peripheral blood were measured by ELISA at 20, 40 and 60 minutes after ischemia, and the pathological changes of ischemic myocardium were observed. Results: The level of MCP-1 in peripheral blood of each group increased gradually with the prolongation of ischemia, the level of MCP-1 in peripheral blood increased significantly in ischemic group and sham operation group (P <0.05) There was no significant difference in the level of MCP-1 between the two groups (P> 0.05). The levels of MCP-1 in middle and high dose groups were significantly decreased at 40min and 60min after ischemia (P <0.05) The level of MCP-1 in high-dose group was lower than that in middle-dose group (P <0.05) at mid-dose and high-dose group PFC can reduce the histopathological changes of ischemic myocardium. Conclusion: Intraperitoneal injection of PFC can reduce the level of MCP-1 in the peripheral blood of rats with myocardial ischemia and play a protective role in ischemic myocardium.