Change in Shape and Crystal Structure of HAP Nanoparticles during Absorption into Cell

• 作 者: 扈盛 李世普
• 机 构: Biomedical Materials and Engineering Center Wuhan University of Technology,Biomedical Materials and
• 出 处: Journal of Wuhan University of Technology(Materials Science
• 发表日期: 2007年03期
• 关键词: degraded cytoplasm hydroxyapatite Absorption incubated biomaterial minutes attac

The change of hydroxyapatite (HAP) nanoparticles in shape and crystal structure after endocytosis into cancer cells was studied. BEL7402 cells were incubated with HAP nanoparticles for 2 hour,8 hours,20 hours,respectively. Then,the cells were collected and viewed under a transmission electronic microscope (TEM). Electronic diffraction (ED) attached to TEM was used to detect the properties of the particles. The results show that HAP particles in the cytoplasm can be degraded in cytoplasm. The degradation process is prolonged by more than 20 hours. Thus,it is concluded that HAP nanoparticles would be degraded after kill cells or delivery gene.

The change of hydroxyapatite (HAP) nanoparticles in shape and crystal structure after endocytosis into cancer cells was studied. BEL7402 cells were incubated with HAP nanoparticles for 2 hour, 8 hours, 20 hours, respectively. Then, the cells were collected and viewed under a Transmission electronic microscope (TEM). Electronic diffraction (ED) attached to TEM was used to detect the properties of the particles. The results show that HAP particles in the cytoplasm can be degraded in cytoplasm. The degradation process is prolonged by more than 20 hours Thus, it is said that HAP nanoparticles would be degraded after kill cells or delivery gene.