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首先对４１种人和小鼠的Ｔ细胞受体β链可变基因编码肽段（Ｖβ）的氨基酸序列进行多序列对准，就Ｖβ之第四高变区（ＨＶ４）片段进行比较，分析与超抗原毒素休克综合征毒素－１（ＴＳＳＴ－１）结合的四种Ｖβ（小鼠Ｖβ３、Ｖβ１５、Ｖβ１７和人Ｖβ２）之ＨＶ４序列内是否存在特定的氨基酸残基排列模式。结果发现：小鼠Ｖβ３和Ｖβ１７的ＨＶ４具有特异的ＲＦＳＡＸＣＸＳＮＳ模式，而小鼠Ｖβ１５和人Ｖβ２的ＨＶ４则含独特的ＫＦＸＩＸＨ模式。提示：与ＴＳＳＴ－１结合的四种Ｖβ所对应的Ｔ细胞识别表位可能不止一个。
Firstly, the amino acid sequences of the peptide encoding the variable region of β-chain of the T cell receptor (Vβ) of 41 human and mouse were aligned by multiple sequence to compare the HV4 segment of Vβ. Whether there is a specific pattern of amino acid residues in the HV4 sequences of the four Vβs (mouse Vβ3, Vβ15, Vβ17 and human Vβ2) to which the superantigen Toxin Shock Syndrome Toxin-1 (TSST-1) binds. As a result, it was found that HV4 of mouse Vβ3 and Vβ17 has a specific RFSAXCXSNS pattern, whereas HV4 of mouse Vβ15 and human Vβ2 have a unique KFXIXH pattern. Tip: The four Vβ binding to TSST-1 may correspond to more than one T cell recognition epitope.