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采用Suzuki偶合方法合成了含羧酸基团的新型阴离子型水溶性绿光的9,9’-二丙酸钠芴(DPF)和噻吩(TH)共聚物,聚合物分子量约为9000左右,具有较好的水溶性(5 mg/mL).研究了共聚物在不同pH水溶液中的荧光性质,结果表明当pH<4时,羧基以COOH形式存在,造成共聚物溶解度降低并产生聚集,荧光减弱;当pH>4时,所含羧基解离程度增加,水溶性增加,链间聚集减少,至其荧光强度增大.在磷酸盐缓冲溶液(PBS)中,加入了不同电性蛋白质,结果表明通过静电吸引的作用,带正电荷的组蛋白能更有效猝灭共聚物荧光,其最低的猝灭浓度可达到5×10-8 mol/L.
A novel anionic water-soluble sodium 9,9’-dipropionate fluorene (DPF) and thiophene (TH) copolymer containing carboxylic acid groups was synthesized by Suzuki coupling method. The molecular weight of the polymer is about 9000, (5 mg / mL) .The fluorescence properties of the copolymer in different pH aqueous solutions were studied.The results showed that when the pH value is less than 4, the carboxyl groups exist in the form of COOH, resulting in the decrease of the solubility and aggregation of the copolymer and the decrease of the fluorescence When pH> 4, the degree of dissociation of the carboxyl groups increased, water solubility increased, the inter-chain aggregation decreased, and its fluorescence intensity increased.Under the phosphate buffer solution (PBS), different electrical proteins were added and the results showed that By electrostatic attraction, the positively charged histone can quench the copolymer fluorescence more effectively, and the lowest quenching concentration can reach 5 × 10-8 mol / L.