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目的:观察西洛他唑对糖尿病大鼠视网膜细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)表达的影响,并探讨其可能的作用机制。方法:以链脲佐菌素(STZ)诱发大鼠糖尿病。大鼠被随机分为正常对照组、糖尿病模型对照组、西洛他唑高剂量(27 mg.kg-1.d-1)和低剂量(9 mg.kg-1.d-1)组。西洛他唑治疗12周后测定血糖和糖化血红蛋白(HbAlc);利用RT-PCR和Western blot技术检测视网膜组织中ICAM-1,VCAM-1,过氧化物酶体增殖物激活受体γ(PPAR-γ)mRNA和蛋白质的表达。结果:与糖尿病模型对照组相比,西洛他唑能显著降低ICAM-1和VCAM-1 mRNA以及蛋白表达水平;西洛他唑治疗后,PPAR-γmRNA和蛋白表达水平明显提高。结论:西洛他唑降低糖尿病大鼠视网膜ICAM-1和VCAM-1表达的作用可能与上调PPAR-γ有关。
Objective: To observe the effect of cilostazol on the expression of ICAM-1 and VCAM-1 in retina of diabetic rats and its possible mechanism. Methods: Diabetic rats were induced by streptozotocin (STZ). Rats were randomly divided into normal control group, diabetic model control group, cilostazol high dose (27 mg.kg-1.d-1) and low dose (9 mg.kg-1.d-1) group. The levels of ICAM-1, VCAM-1, peroxisome proliferator-activated receptor gamma (PPAR) in retinal tissue were detected by RT-PCR and Western blot after cilostazol treatment for 12 weeks. -γ) mRNA and protein expression. Results: Compared with the diabetic model control group, cilostazol could significantly decrease the mRNA and protein expression of ICAM-1 and VCAM-1. After cilostazol treatment, the expression of PPAR-γmRNA and protein were significantly increased. Conclusion: The effect of cilostazol on the expression of ICAM-1 and VCAM-1 in the retina of diabetic rats may be related to the increase of PPAR-γ.