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通过高特异性、高敏感性的抗原抗体反应,应用透射比浊法,对86例健康人红细胞膜(RBC_m)载脂蛋白(apo)A_1、B及胆固醇作了测定分析,其中apoB,A_1的参考范围分别为23.25±4.02×10~(12)mg/cell、7.32±1.95×10~(12)mg/cell,胆固醇为0.28±0.44×10~(12)mmol/cell。其批内变异系数均<5％。膜胆固醇与apoA_1、B均存在显著相关性。apoA_1与apoB亦有相关性。显然,膜载脂蛋白A_1、B均与膜胆固醇的代谢有密切关系。即提示其在RBC_m与血浆脂蛋白、胆固醇交换中起到转运并维持膜胆固醇的稳定性作用。另外对40例糖尿病人(DM)的红细胞膜的apoA_1、B及胆固醇作了相应测定。结果表明RBC_m的apoA_1有显著性增高,spoB有显著性降低,而膜胆固醇无明显变化。而且其膜胆固醇/膜apoA_1有显著性降低,而膜胆固醇/膜apoB有显著性增高。
86 cases of healthy human erythrocyte membrane (RBC_m) apolipoproteins A_1, B and cholesterol were assayed by high specificity and high sensitivity of antigen-antibody reaction. The levels of apoB, A_1 The reference ranges were 23.25 ± 4.02 × 10 ~ (12) mg / cell, 7.32 ± 1.95 × 10 ~ (12) mg / cell and cholesterol 0.28 ± 0.44 × 10 ~ (12) mmol / cell respectively. Within the batch coefficient of variation <5%. Membrane cholesterol and apoA_1, B are significant correlation. ApoA_1 is also related to apoB. Obviously, the membrane apolipoprotein A_1, B are closely related with the metabolism of membrane cholesterol. That is to say it plays a role in the transport of RBC_m and plasma lipoprotein, cholesterol exchange and maintain the stability of membrane cholesterol. In addition, 40 cases of diabetes mellitus (DM) erythrocyte membrane apoA_1, B and cholesterol were measured accordingly. The results showed that apoA_1 in RBC_m increased significantly, spoB decreased significantly, but no significant changes in membrane cholesterol. And its membrane cholesterol / membrane apoA_1 significantly reduced, while the membrane cholesterol / membrane apoB increased significantly.