Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27

• 作 者: Jin-Shui Zhu Long Wang Guo-Qiang Cheng Qin Li Zu-Ming Zhu Li Zhu
• 机 构: Department of Gastroenterology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Sh
• 出 处: 世界胃肠病学杂志（英文版）
• 发表日期: 2005年47期
• 关键词: Gastric cancer Human mutant p27 Cell lineMKN-45

AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%)compared to the control group (3.12±0.13%, P ＜ 0.01).CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.