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我国食品卫生标准规定,饮料中糖精钠的含量不得超过0.15g/kg, 苯甲酸、山梨酸不得超过0.2g/kg。采用高效液相色谱法,饮料经过滤,用10～12分钟能同时测定这三种成分。本法回收率为97～110％,标准偏差0.29,变异系数0.038.含量在0.1～20μg范围内,线性关系良好。 1.仪器与试剂:美国Waters246型高效液相色谱仪,441型紫外检测器,检测波长254mm。甲醇:分析纯,重蒸;0.02M醋酸铵溶液;0.1mg/ml糖精钠,苯甲酸,山梨酸混合标准液0.1mg/ml糖精钠,苯甲酸和0.05mg/ml山梨酸混合标准液。以上溶液均用重蒸水配制。 2.实验条件:Mbondapak C~T_84×300mm不锈钢色谱柱,流动相,甲醇/0.02M醋酸铵(35/65),1ml/min,纸速0.5m/min。 3.方法与结果: (1)标准曲线法:首先分别测出糖精钠,苯
China’s food hygiene standards, the content of saccharin sodium drinks shall not exceed 0.15g / kg, benzoic acid, sorbic acid shall not exceed 0.2g / kg. Using high-performance liquid chromatography, the beverage is filtered, and the three components can be simultaneously measured with 10 to 12 minutes. The recovery rate of this method was 97-110%, the standard deviation was 0.29 and the coefficient of variation was 0.038. The linearity was good in the range of 0.1 ~ 20μg. 1. Instruments and reagents: the United States Waters246 HPLC, 441 UV detector, detection wavelength 254mm. Methanol: analytical grade, double distilled; 0.02M ammonium acetate solution; 0.1mg / ml sodium saccharin, benzoic acid, sorbic acid mixed standard 0.1mg / ml sodium saccharin, benzoic acid and 0.05mg / ml sorbic acid mixed standard solution. The above solutions are prepared with distilled water. 2. Experimental conditions: Mbondapak C ~ T_84 × 300mm stainless steel column, mobile phase, methanol / 0.02M ammonium acetate (35/65), 1ml / min, paper speed 0.5m / min. 3 methods and results: (1) standard curve method: first were measured sodium saccharin, benzene