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以杂种落叶松(兴10×日13)成熟合子胚为外植体,通过不同培养基与激素的组合进行实验,最终形成完整落叶松植株,根据试验结果建立并优化了杂种落叶松成熟合子胚诱导植株再生的体系。结果表明:BM培养基有利于杂种落叶松不定芽的诱导,诱导率达60.7%,且在BM+1.5 mg·L~(-1)TDZ条件下诱导率最高且为67.73%;BM培养基能促进不定芽增殖,增殖系数达4.48,且在BM+1.0 mg·L~(-1)TDZ条件下增殖系数最高且为5.23;不定芽在1/2BM+0.5 mg·L~(-1)TDZ伸长比率最高且为205.72%,同时,在1/2BM培养基中添加2 g·L~(-1)的活性炭也能促进伸长;当不定芽在BM+0.05 mg·L~(-1)6-BA+0.2 mg·L~(-1)NAA的条件下培养时,抽茎率最高,为73.96%;不定芽在1/2BM+0.5 mg·L~(-1)IBA+1.0 mg·L~(-1)NAA条件下诱导生根的生根率最高,为29.85%。
The mature larch embryos of hybrid larch (Hing 10 × day 13) were used as explants, and the complete larch plant was formed through the combination of different media and hormones. Based on the experimental results, the mature larch embryo was established and optimized System for inducing plant regeneration. The results showed that: BM medium was conducive to the induction of adventitious buds of hybrid Larch, the induction rate was 60.7%, and the highest induction rate was 67.73% under the condition of BM + 1.5 mg · L -1 TDZ; BM medium The multiplication coefficient of adventitious buds was 4.48, and the highest proliferation coefficient was 5.23 under the condition of BM + 1.0 mg · L -1 TDZ. The number of adventitious buds was highest at 1 / 2BM + 0.5 mg · L -1 TDZ The highest elongation was 205.72%. At the same time, adding 2 g · L -1 activated carbon in 1 / 2BM medium also promoted elongation. When adventitious buds were cultured in the medium of BM + 0.05 mg · L -1 ) Buds were the highest, accounting for 73.96% when cultured with 6-BA + 0.2 mg · L -1 NAA. Adventitious buds were cultured in 1 / 2BM + 0.5 mg · L -1 IBA + 1.0 mg The rooting rate of induced rooting under the condition of L ~ (-1) NAA was the highest (29.85%).