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[目的]研究大黄对重症急性胰腺炎(SAP)大鼠肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、单核细胞趋化因子1(MCP-1)的影响,探讨大黄治疗SAP的作用机制。[方法]采用胆胰管逆行注射5%牛磺胆酸钠法建立大鼠SAP模型。将SD大鼠随机分为假手术组、SAP组、大黄治疗(大黄)组。观察各组不同时间点血清淀粉酶、TNF-α、IL-6、MCP-1水平及胰腺组织中TNF-α、IL-6、MCP-1的mRNA水平。[结果]与假手术组比较,SAP组血清淀粉酶、TNF-α、IL-6、MCP-1水平均明显升高,胰腺组织中TNF-α、IL-6、MCP-1mRNA水平亦明显增加(均P<0.01);大黄组与SAP组比较,血清淀粉酶、TNF-α、IL-6、MCP-1水平及胰腺组织中TNF-α、IL-6、MCP-1mRNA均明显降低(均P<0.01)。[结论]大黄治疗SAP的机制可能是通过下调炎症递质的表达而减轻SAP的炎症反应。
[Objective] To study the effects of rhubarb on tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) in rats with severe acute pancreatitis (SAP). Explore the mechanism of rhubarb treatment SAP. [Methods] Rat SAP model was established by retrograde injection of 5% sodium taurocholate into the bile ducts. SD rats were randomly divided into sham operation group, SAP group, rhubarb treatment (rhubarb) group. The levels of serum amylase, TNF-α, IL-6, MCP-1 and the mRNA levels of TNF-α, IL-6 and MCP-1 in pancreatic tissue were observed at different time points. [Results] Compared with the sham group, the levels of serum amylase, TNF-α, IL-6 and MCP-1 were significantly higher in the SAP group, and the levels of TNF-α, IL-6 and MCP-1 mRNA in the pancreas were also significantly increased. (P <0.01); Compared with SAP group, serum amylase, TNF-α, IL-6, MCP-1 levels and pancreatic tissue TNF-α, IL-6, MCP-1 mRNA were significantly lower in rhubarb group than in SAP group (both P<0.01). [Conclusion] The mechanism of Rhubarb treating SAP may be to reduce the inflammatory response of SAP by down-regulating the expression of inflammatory mediators.