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背景:目前在探讨骨缺损的物理和药物方法治疗的效果、检验手术方式的有效性、以及评价骨替代材料在骨缺损修复中所起的作用时,尚缺少一种客观的、标准的动物模型。目的:为骨不连的实验研究建立一种客观的动物实验模型。设计:制作兔骨不连模型的验证性实验。单位:深圳市人民医院骨科。材料:取普通级纯种新西兰大白兔20只,体质量(2.5±0.5)kg,6～8月龄,雌雄不限。方法:实验于1999-05/08在深圳市人民医院动物实验中心完成。选取纯种新西兰大白兔20只,在前臂桡骨中段截除1.5cm骨段(包括骨膜),骨断端用骨蜡封闭髓腔,10周后经大体标本、病理组织学及X射线检查确定骨不连形成情况。主要观察指标:兔前臂桡骨骨缺损模型大体观察及病理组织学和放射学检查结果。结果:纳入白兔20只(40侧桡骨)均进入结果分析。①兔骨缺损区大体观察:10周后骨缺损区可见纤维瘢痕组织填充,无骨性连接,骨蜡未吸收,骨端硬化,髓腔闭塞,两侧断端有少量骨痂形成,骨缺损范围为0.8～1.2cm。②兔骨缺损区病理组织学检查:光镜下骨断端髓腔封闭,有软骨细胞及骨细胞,细胞呈无序排列,纤维膜覆盖,缺损区为纤维瘢痕组织。③兔骨缺损区放射学检查:于10周X射线拍片见骨缺损区无骨性连接,断端硬化,髓腔封闭,两侧断端有少量骨痂形成,骨痂形成不规则。结论:实验所建立的动物模型,具有骨不连的病理改变,符合骨不连的要求,反映了骨不连的一般特征,可以认为是一种可靠而实用的实验性骨不连动物模型。
BACKGROUND: At present, there is a lack of an objective and standard animal model for examining the effects of physical and medical treatment of bone defects, testing the effectiveness of surgical procedures, and evaluating the role of bone substitutes in the repair of bone defects. . Objective: To establish an objective experimental animal model for the experimental study of nonunion. DESIGN: AUTHENTICITY EXPERIMENT TO MAKE THE CONJUGATE MODEL OF TUBULAR BONE. Unit: shenzhen people’s hospital orthopedics. MATERIALS: Twenty normal New Zealand white rabbits were obtained. Body weight (2.5 ± 0.5) kg, 6-8 months old, male or female. Methods: The experiment was performed at Animal Experimental Center of Shenzhen People’s Hospital from May 1999 to August 08. Twenty rabbits of pure New Zealand white rabbits were selected, and 1.5 cm bone segments (including periosteum) were cut off in the middle of the forearm radius. The bone marrow was closed with bone wax at the fracture site. After 10 weeks, the bone samples were determined by gross pathology, histopathology and X-ray examination Not formed. MAIN OUTCOME MEASURES: The model of radial forearm in rabbits was observed and histopathological and radiological findings were observed. Results: Twenty rabbits (40 sides) were included in the results analysis. ① Rabbit bone defect area in general observation: 10 weeks after the bone defect visible fibrous scar tissue filling, no bone connection, bone wax absorption, bone sclerosis, medullary cavity occlusion, a small amount of callus formation on both sides of the fracture, bone defects The range is 0.8 ~ 1.2cm. ② Pathological examination of rabbit bone defect area: Under the light microscope, the medullary cavity was closed with chondrocytes and osteocytes, the cells were arranged in disorder, the fibrous membrane was covered and the defect area was fibrous scar tissue. ③ Radiological examination of rabbit bone defect area: X-ray film at 10 weeks showed no bone connection in the defect area, with the end-side sclerosis and the medullary cavity closed. There was a small amount of callus formation on both sides of the broken end. The callus formation was irregular. Conclusion: The animal model established by the experiment has the pathological changes of nonunion and meets the requirements of nonunion. It reflects the general characteristics of nonunion and can be regarded as a reliable and practical experimental nonunion model.