苹果属植物(砧木 M.9)愈伤组织的器官分化

• 作 者: 陈维伦 杨善英 王洪新 崔澂
• 机 构: 中国科学院植物研究所生理生化研究室,中国科学院植物研究所生理生化研究室,中国科学院植物研究所生理生化研究室,中国科学院植物研究所生理生化研究室,
• 出 处: Journal of Integrative Plant Biology
• 发表日期: 1979年02期
• 关键词: 愈伤组织 M.9 苹果属 器官分化 诱导愈伤 分化培养基 继代培养 切段 幼胚 无菌苗

近年来有关木本植物愈伤组织器官分化的报道正在逐渐增多。关于苹果属植物器官分化的研究已有些报道。Whiteley和 Abbott初步报道苹果幼胚愈伤组织经继代培养后能分化根和芽,但幼年和成年期的茎愈伤组织仍不能分化芽。我们用无菌培养的苹果属植物(砧木 M.9)的茎切段作材料,诱导愈伤组织形成,继代培养后能分化成芽和产生植株,并有较高的分化频率。

In recent years, reports of organotypic differentiation of woody plant calluses are gradually increasing. Research on the differentiation of apple genus organ has been reported. Whiteley and Abbott initially reported that callus from apple immature embryos could differentiate into root and bud after subculture, but stem callus did not differentiate in juvenile and adult. We used the stalks of the apricot (rootstock M.9), which were cultured in asepsis, to induce the formation of callus. After subculture, we can differentiate into shoots and plants and have higher differentiation frequency.