论文部分内容阅读
目的研究与分析腺瘤性结肠息肉病基因启动子区甲基化表现状态及其在宫颈癌中发生及发展的作用及意义。方法选取本院所收治的120例宫颈鳞癌患者及30例正常宫颈上皮组织进行研究，并采用甲基化特异性聚合酶链反应法检测其组织中腺瘤性结肠息肉病基因启动子区甲基化状态，分析并比较。结果经检测发现，30例正常宫颈上皮组织中未检测到腺瘤性结肠息肉病基因甲基化片段，而宫颈癌组织中，其腺瘤性结肠息肉病基因甲基化阳性率为65.0%(78/120)；两组阳性率比较(<0.05)。宫颈癌组织中，其不同临床分期间腺瘤性结肠息肉病基因甲基化阳性率比较(0.05)。结论宫颈癌形成过程中，腺瘤性结肠息肉病基因启动子区异常甲基化是早期且较为频繁发生的事件，因此，其可能参与到宫颈癌的发生与发展中，进而为临床疾病诊断及预后判断提供重要的参考价值。“,”Objective Research and Analysis adenomatous polyposis coli gene promoter methylation status and its role and significance of the performance of the occur ence and development in cervical cancer.Methods Select this institute admit ed 120 cases of cervical squamous cel carcinoma and 30 cases of normal cervical epithelial tissue research,and to detect their organization adenomatous polyposis coli gene promoter methylation using methylation-specific polymerase chain reaction state,analyzed and compared.Results After testing found 30 cases of normal cervical epithelium adenomatous polyposis coli gene methylation was not detected fragments,and cervical carcinoma,which adenomatous polyposis coli gene methylation positive rate was 65.0%(78/120);positive rates of the two groups ( <0.05).Cervical carcinoma,the positive rate of its dif erent adenomatous polyposis coli gene methylation during clinical comparison ( 0.05).Conclusion Cervical cancer formation,adenomatous polyposis coli gene promoter methylation is abnormal event occurs earlier and more frequently,and therefore,it may be involved in the occur ence and development of cervical cancer,and thus for the clinical diagnosis of disease and prognosis judgment provides important reference value.