论文部分内容阅读
目的比较并探讨两种聚合酶链反应技术在单纯疱疹病毒性脑炎（HSE）早期快速诊断中的应用价值。方法选择30例临床确诊的病毒性脑炎患者，分别运用由两种不同引物组成的聚合酶链反应（PCR）和巢式聚合酶链反应（nested－PCR）测定脑脊液中的单纯疱疹Ⅰ型病毒（HSV－Ⅰ）DNA，另选择25例非病毒性脑炎患者的脑脊液作为对照组。结果采用PCR扩增检测，检测阳性率为67％；采用巢式PCR扩增检测，检测阳性率为40％；25例非病毒性脑炎患者的脑脊液（对照组）经PCR和巢式PCR扩增，均未检出HSV-ⅠDNA。结论无论是PCR还是巢式PCR都具有高度的特异性；而在灵敏性方面，前者不如后者。表明PCR技术可有效地提高HSE的早期诊断水平，尤其是巢式PCR方法更有价值。
Objective To compare and explore the value of two kinds of polymerase chain reaction (PCR) in the early rapid diagnosis of herpes simplex virus encephalitis (HSE). Methods Thirty patients with clinically diagnosed viral encephalitis were enrolled in this study. Herpes simplex virus type 1 (CSFV) in cerebrospinal fluid (CSF) was determined by polymerase chain reaction (PCR) and nested-PCR (HSV-Ⅰ) DNA, and the other 25 cases of non-viral encephalitis in patients with cerebrospinal fluid as a control group. Results The positive rate was 67% by PCR amplification. The positive rate was 40% by nested PCR amplification. The cerebrospinal fluid (control group) of 25 non-viral encephalitis patients was amplified by PCR and nested PCR Increased, no detection of HSV-Ⅰ DNA. Conclusion Both PCR and nested PCR are highly specific; whereas in terms of sensitivity, the former is not as good as the latter. It is indicated that PCR technology can effectively improve the early diagnosis of HSE, especially the nested PCR method is more valuable.