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目的:探讨VEGF-D,CD105在胰腺癌发生、发展及转移过程中的意义。方法:采用免疫组织化学S-P法,检测VEGF-D,CD105在53例胰腺癌组织(胰腺癌组)及10例正常胰腺组织(对照组)中的表达,计数MVD,并与临床病理学资料进行分析比较。结果:胰腺癌组织中VEGF-D的阳性表达明显高于正常胰腺组织(P<0.05)。VEGF-D的表达与胰腺癌的临床分期及淋巴结有无转移有关(P<0.05)。胰腺癌中,CD105标记的微血管密度(MVD)明显高于非肿瘤性胰腺组织。CD105的表达水平与胰腺癌临床分期及病理分级有关。胰腺癌组织中CD105抗体标记的微血管密度(MVD)结果显示:在有淋巴结转移的癌组织中,其MVD值高于无淋巴结转移组有显著统计学意义(P<0.05)。VEGF-D阳性表达组的MVD显著高于VEGF-D表达阴性组的MVD(P<0.05);CD105在胰腺癌中的表达与VEGF-D的表达呈正相关(P<0.05)。结论:VEGF-D的表达与胰腺癌的血管生成有关。MVD值有可能作为判断胰腺癌生物学行为的潜在指标。
Objective: To investigate the significance of VEGF-D and CD105 in the pathogenesis, development and metastasis of pancreatic cancer. Methods: Immunohistochemical SP method was used to detect the expression of VEGF-D and CD105 in 53 cases of pancreatic cancer (pancreatic cancer) and 10 cases of normal pancreatic tissue (control group). MVD was counted and compared with clinicopathological data analyse and compare. Results: The positive expression of VEGF-D in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues (P <0.05). The expression of VEGF-D was correlated with the clinical stage of pancreatic cancer and lymph node metastasis (P <0.05). In pancreatic cancer, CD105-labeled microvessel density (MVD) was significantly higher than that of non-neoplastic pancreatic tissue. The expression of CD105 is related to the clinical stage and pathological grade of pancreatic cancer. The results of MVD in CD105 antibody in pancreatic cancer showed that MVD in lymph node metastasis was significantly higher than that in non-lymph node metastasis (P <0.05). The MVD in VEGF-D positive group was significantly higher than that in VEGF-D negative group (P <0.05). The expression of CD105 in pancreatic cancer was positively correlated with the expression of VEGF-D (P <0.05). Conclusion: The expression of VEGF-D is related to the angiogenesis of pancreatic cancer. MVD may be used as a potential indicator of pancreatic cancer biological behavior.