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本文用组织化学的方法,探讨不同地区周期型马来丝虫是否存在种下分型的问题。这对马来丝虫病防治措施的进一步完善,对该病最适诊断方法的选择等将提供重要的理论依据。 材料与方法 1 微丝蚴来源 分别从实验感染乐山(L)、独山(D)和建阳(J)三株马来丝虫的长爪沙鼠(前者虫株引种于四川省寄生虫病防治研究所,后两者虫株自建)各6～8只的腹腔,抽取含微丝蚴的腹腔液涂片。 2 碱性磷酸酶(AKP)和酸性磷酸酶(ACP)染色 AKP染色按照Gomori的钙钴法。ACP染色参照Chalifoux等的方法;4℃纯丙酮固定涂片1min→萘酚AS—TR磷酸酯作底物,pH5.0,37℃孵育1h→复染→脱水→透明→封片。对照组分为:
In this paper, the use of histochemical methods to explore different regions of the periodic Malayan worm there is the problem of sub-type. This further improvement of malayian filariasis prevention and control measures will provide an important theoretical basis for the selection of the optimal diagnostic method for the disease. MATERIALS AND METHODS 1 The sources of microfilariae were isolated from experimental gerbils infected with three strains of malayian filariasis from Leshan (L), Dushan (D) and Jianyang (J) Prevention and Control Institute, the latter two self-built insect strains) of each 6 to 8 abdominal cavity, taking the peritoneal fluid containing microfilaria smear. 2 Alkaline phosphatase (AKP) and acid phosphatase (ACP) staining AKP staining was performed according to Gomori’s calcium-cobalt method. ACP staining with reference to Chalifoux and other methods; 4 ℃ pure acetone fixed smear 1min → naphthol AS-TR phosphate as a substrate, pH5.0, 37 ℃ incubated 1h → counterstain → dehydration → transparent → sealing. Control components are: