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目的 观察重组质粒VR10 12 -Pf12DNA直接免疫接种诱导BALB/c小鼠所产生的免疫应答 ,分析Pf12基因的抗原性和作为疫苗抗原候选分子的可能性。方法 构建重组质粒VR10 12 -Pf12 ,直接免疫BALB/c小鼠 ,通过NK细胞杀伤活性、脾T淋巴细胞增殖水平、ELISA、体外抑虫试验测定 ,观察其诱导的细胞和体液应答以及体外抑虫效果。结果 重组质粒VR10 12 -Pf12免疫BALB/c小鼠 ,NK细胞杀伤活性、脾T淋巴细胞增殖水平明显高于对照组 (P <0 0 1) ,诱导小鼠产生的抗体水平明显增高 (P <0 0 1) ,免疫血清在体外能抑制恶性疟原虫的生长、发育。结论 重组质粒VR10 12 -Pf12DNA直接免疫接种诱导BALB/c小鼠产生一定水平的体液和细胞免疫应答 ,其免疫血清在体外对恶性疟原虫的生长、发育有明显的抑制作用。
Objective To observe the immune response of BALB / c mice induced by direct immunization of recombinant plasmid VR10 12 -Pf12 DNA and to analyze the antigenicity of Pf12 gene and the possibility of being candidate antigen of vaccine antigen. Methods The recombinant plasmids VR10 12 -Pf12 were constructed and directly immunized BALB / c mice. The cytotoxicity of NK cells and proliferation of splenic T lymphocytes were assayed by ELISA and in vitro inhibition test. effect. Results The NK cell activity and splenic T lymphocyte proliferation of BALB / c mice immunized with the recombinant plasmid VR10 12 -Pf12 were significantly higher than those of the control group (P <0.01), and the antibody levels of the induced mice were significantly increased (P < 0 0 1), immune serum can inhibit the growth and development of Plasmodium falciparum in vitro. Conclusion The direct immunization of VR10 12 -Pf12 DNA by recombinant plasmid induces a certain level of humoral and cellular immune response in BALB / c mice. The immune serum has a significant inhibitory effect on the growth and development of P. falciparum in vitro.