论文部分内容阅读
目的:观察脾虚4号方对功能性腹泻脾虚证大鼠模型的作用及对大鼠结肠组织胰高血糖素肽1受体表达的影响,探讨其作用机制。方法:高乳糖加小平台站立法制备功能性腹泻脾虚证大鼠模型,观察大鼠的腹泻指数和体重变化,比色法检测血清乳酸、淀粉酶、尿D-木糖含量变化。脾虚4号方治疗功能性腹泻脾虚证模型大鼠后,分别用免疫组织化学法和蛋白质印迹法检测大鼠结肠组织胰高血糖素肽1受体的表达情况。结果:功能性腹泻脾虚证模型组大鼠较正常组大鼠的腹泻指数增加,体重增加减缓。模型组大鼠较正常组大鼠的血清乳酸含量升高、血清淀粉酶活力降低、尿D-木糖含量降低。模型组大鼠结肠组织的GLP-1R表达水平较正常组降低且有统计学意义(P<0.01);脾虚4号方治疗组GLP-1R表达水平较模型组升高且有统计学意义(P<0.01)。结论:成功复制功能性腹泻脾虚证大鼠模型。脾虚4号方对功能性腹泻脾虚证大鼠有一定的治疗作用,脾虚4号方使功能性腹泻脾虚证大鼠结肠胰高血糖素肽1受体表达升高。推断胰高血糖素肽1受体可能与脾虚4号方对功能性腹泻脾虚证的治疗有相关性。
Objective: To observe the effect of spleen deficiency recipe 4 on functional diarrhea spleen deficiency rat model and its effect on the expression of glucagon peptide 1 receptor in colon tissue of rats, and to explore its mechanism. Methods: A rat model of Functional Diarrhea and Spleen Deficiency Syndrome was established by high lactose plus small platform. The diarrhea index and body weight of rats were observed. The contents of serum lactic acid, amylase and urine D-xylose were detected by colorimetry. Spleen deficiency prescription 4 treatment of functional diarrhea spleen deficiency model rats, immunohistochemistry and Western blot were used to detect the expression of glucagon peptide 1 receptor in rat colon tissue. Results: Diarrhea index and weight gain of rats in functional diarrhea and spleen deficiency model group were higher than those in normal rats. Compared with normal group, serum lactate level increased, serum amylase activity decreased and urinary D-xylose content decreased in model group rats. The expression of GLP-1R in colon tissue of model group was lower than that of normal group (P <0.01), and the expression of GLP-1R in model group was significantly higher than that of model group (P <0.01). Conclusion: The successful replication of functional diarrhea spleen deficiency rat model. Spleen deficiency on the 4th party of functional diarrhea spleen deficiency rats have a certain therapeutic effect, spleen deficiency on the 4th party to functional diarrhea spleen deficiency in rats colon glucagon peptide 1 receptor expression increased. It is concluded that the glucagon peptide 1 receptor may be related to the treatment of spleen deficiency type 4 on functional diarrhea and spleen deficiency syndrome.