论文部分内容阅读
目的:分析表皮生长因子受体家族中的ErbB-3受体和氨基酸转运蛋白L型氨基酸转运体-1(LAT-1)在临床前列腺癌组织标本中的表达和定位,以及二者之间可能存在的相关性。方法:对45例前列腺癌组织标本,10例良性前列腺增生组织(BPH)标本和9例正常前列腺标本的石蜡切片采用免疫组织化学SABC方法染色并进行分析。结果:在45例前列腺癌组织中有33例(73.3%)细胞质或细胞核呈现ErbB-3染色阳性;10例BPH标本中,仅有1例出现阳性反应(P<0.001);正常前列腺组织中未见到阳性表达(P<0.001)。而LAT-1在前列腺癌组织中的表达阳性率高于ErbB-3,为40例(88.9%),表达于细胞膜和或细胞质中,明显高于正常组织以及BPH组织(P<0.05)。LAT-1在正常组织和BPH组织中无阳性表达。ErbB-3和LAT-1在前列腺癌组织中的表达均与Glean评分分级无关。在前列腺癌组织中ErbB-3和LAT-1的表达存在正相关性(r=0.426,P<0.05)。结论:在前列腺组织中的ErbB-3和LAT-1阳性表达和定位能够区分恶性肿瘤与BPH和正常前列腺组织,有利于前列腺癌的诊断和生物学的预测。
OBJECTIVE: To analyze the expression and location of ErbB-3 receptor and amino acid transporter L-amino acid transporter-1 (LAT-1) in the epidermal growth factor receptor family in clinical specimens of prostate cancer and their possible relationship Existing relevance. Methods: Paraffin sections of 45 specimens of prostate cancer, 10 specimens of benign prostatic hyperplasia (BPH) and 9 specimens of normal prostate were stained and analyzed by immunohistochemical SABC method. Results: In 45 cases of prostate cancer, 33 cases (73.3%) showed positive ErbB-3 staining in cytoplasm or nucleus; only 1 of 10 BPH specimens showed positive reaction (P <0.001); in normal prostate tissue Positive expression was seen (P <0.001). The positive expression rate of LAT-1 in prostate cancer tissue was higher than that of ErbB-3 (40 cases, 88.9%), which was significantly higher than that in normal tissues and BPH tissues (P <0.05). LAT-1 was not expressed in normal tissues and BPH tissues. The expressions of ErbB-3 and LAT-1 in prostate cancer tissues were not related to the Glean score. There was a positive correlation between the expression of ErbB-3 and LAT-1 in prostate cancer tissues (r = 0.426, P <0.05). CONCLUSIONS: The positive expression and localization of ErbB-3 and LAT-1 in prostate tissue can distinguish malignant tumor from BPH and normal prostate tissue, which is helpful for the diagnosis and biological prediction of prostate cancer.