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Objective:To analyze the effect of black soybean natto treatment on the recovery of the reproduction system in hypercholesterolemic male mice model.Methods: Thirty male mice were divided into five groups, they were normal mice (K-), a hypercholesterolemic mice model (K+), a hypercholesterolemic mice model with 200 mg/kg BW of natto extract (P1), a hypercholesterolemic mice model treated with 400 mg/kg BB of natto extract (P2), and a hypercholesterolemic mice model treated with 800 mg/kg BB of natto extract (P3). Natto extract was given orally for 30 d.Results: Black soybean natto extract influenced on reproduction system of male hypercholesterolemic mice by increasing the testosterone level and increasing the density and motility of sperm.Conclusion: Black soybean natto extract is promising as hypercholesterolemic therapy for treating the male reproduction system.