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目的: 检测以恶性疟原虫裂殖子表面蛋白1 的17 区片段基因为基础的复合核酸疫苗VR1012/TPA/HG-MSP1-17(分泌性)和VR1012/HG-MSP1-17(非分泌性)诱导小鼠的体液免疫反应。方法: 分别用分泌性与非分泌性核酸疫苗肌注免疫小鼠。用间接ELISA法测定小鼠血清的特异性抗体。结果: 每只小鼠经3 次100 μg/100μl非分泌性核酸疫苗免疫后, BALB/c小鼠和C57BL/6 小鼠均产生明显的抗HG和抗真菌表达的MSP1 17 区蛋白(YMSP119 )抗体。但总体抗体水平不高。BALB/c小鼠经3 次200 μg/100 μl非分泌性核酸疫苗免疫后, 产生较高的抗HG抗体, 但抗MSP1-17 的抗体无明显变化。经3 次200 μg/100 μl分泌性核酸疫苗免疫后, 只产生较低的抗HG抗体。结论: 非分泌性核酸疫苗较分泌性核酸疫苗具有更强的免疫原性
OBJECTIVE: To examine the combined nucleic acid vaccine VR1012 / TPA / HG-MSP1-17 (secreted) and VR1012 / HG-MSP1-17 (non-secreted) based on the gene encoding region 17 of Plasmodium falciparum merozoite surface protein 1 Murine humoral immune response was induced. Methods: Mice were immunized intramuscularly with secreted and non-secreted nucleic acid vaccine respectively. Indirect ELISA was used to determine the serum specific antibody in mice. Results: BALB / c mice and C57BL / 6 mice all developed significant anti-HG and anti-fungal expression of MSP1 17 protein (YMSP119) after 3 times of 100 μg / 100μl non-secreted nucleic acid vaccine. antibody. However, the overall antibody level is not high. BALB / c mice developed high anti-HG antibodies after 3 times of 200 μg / 100 μl non-secreted nucleic acid vaccine, but there was no significant change in antibodies against MSP1-17. After three 200 μg / 100 μl secretory nucleic acid vaccine immunizations, only low anti-HG antibodies were produced. Conclusion: Non-secretory nucleic acid vaccine is more immunogenic than secretory nucleic acid vaccine