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蔬菜灰霉病的灰葡萄孢(Botrytiscinerea)分生孢子经紫外光灯照射,可获得10-4~10-5频率的速克灵抗药性突变体。获得的8个抗性突变体的抗性水平平均EC50值为11.9776μg/ml,EC90值为226.5361μg/ml。抗性突变体的酯酶同工酶港带比其亲本敏感菌株谱带多2~4条,其中Rf=0.38的谱带是抗性菌株所拥有而敏感菌株所共同缺乏的。抗性菌株对速克灵抗药性能稳定遗传,具有与亲本菌株相同的繁殖能力和致病性。突变体和抗性菌株苗期抗、感测定结果表明,对速克灵、多菌灵杀菌剂表现双重抗性,但对灰霉克、利得杀菌剂不存在交互抗性。
Botrytis cinerea conidia of vegetables Botrytis cinerea spores irradiated by UV light can be obtained 10-4 ~ 10-5 frequency of Klinking resistant mutants. The resistance levels of the eight resistant mutants obtained showed an average EC50 value of 11.9776 μg / ml and an EC90 value of 226.5361 μg / ml. Esterase isoenzyme bands of resistant mutants had 2 to 4 bands more than those of their parents, of which the bands with Rf = 0.38 were common to the resistant strains but not to the sensitive ones. The resistant strains are stable and stable to the antibacterial activity of Sukuling and have the same reproductive capacity and pathogenicity as the parental strains. Mutant and resistant strains at seedling anti-sense determination results show that the speed Keling, carbendazim fungicides showed double resistance, but there is no cross-resistance to gray mold grams, benefit fungicides.