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目的:探讨不同浓度蚓激酶(EFE)在体外对LO2细胞株(人正常肝细胞)及软脂酸(PA)诱导的LO2细胞增殖的影响及EFE的最佳作用浓度。方法:用不同浓度(100 u/m L、200 u/m L、400 u/m L、600 u/m L、800 u/m L、1 000 u/m L)EFE作用于LO2细胞,分别在作用6 h、12 h、24 h,通过MTT法检测各组细胞的OD值;用20μg/m L的PA作用LO2细胞24 h,然后用不同浓度(100 u/m L、200 u/m L、400 u/m L、600 u/m L、800 u/m L、1 000 u/m L)的EFE进行干预,并于6 h、12 h、24 h检测细胞增殖情况。结果:200 u/m L、400 u/m L的EFE可促进LO2细胞的增殖,但无统计学意义,高浓度(800 u/m L、1 000 u/m L)的EFE能明显抑制LO2细胞的增殖(P<0.05),且其抑制作用随着时间的延长而增强;低浓度(100 u/m L)的EFE可促进经PA诱导的LO2细胞的增殖,其作用效果显著(P<0.05);200 u/m L、400U/m L的EFE对经PA诱导的LO2细胞的促进作用极显著(P<0.01)。结论:EFE能缓解PA诱导的细胞损伤,对PA诱导的LO2细胞具有保护作用,且其最佳作用浓度为400 u/m L。
Objective: To investigate the effects of different concentrations of lumbrokinase (EFE) on the proliferation of LO2 cells (human normal hepatocytes) and palmitate (PA) -induced LO2 cells in vitro and the optimal concentrations of EFE. Methods: LO2 cells were treated with different concentrations of EFE (100 u / m L, 200 u / m L, 400 u / m L, 600 u / m L, 800 u / m L, 1000 u / m L) The OD value of each group of cells was detected by MTT assay at 6 h, 12 h and 24 h after treatment. LO2 cells were treated with 20 μg / mL PA for 24 h, then treated with different concentrations (100 u / m L, 200 u / m L, 400 u / m L, 600 u / m L, 800 u / m L, 1 000 u / m L). The cell proliferation was measured at 6 h, 12 h and 24 h. Results: EFE at 200 u / m L and 400 u / m L could promote the proliferation of LO2 cells, but there was no statistical significance. EFE at high concentration (800 u / m L, 1000 u / m L) (P <0.05), and its inhibitory effect increased with time. EFE at low concentration (100 u / m L) promoted the proliferation of LO2 cells induced by PA, and its effect was significant (P < 0.05). EFE at 200 u / m L and 400 U / m L significantly promoted the proliferation of LO2 cells induced by PA (P <0.01). CONCLUSION: EFE can relieve PA-induced cell injury and protect PA-induced LO2 cells. The optimal concentration of EFE is 400 u / m L.