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目的探讨超声空化效应破坏兔胶原诱导关节炎滑膜血管翳的最适超声辐照及微泡条件。方法建立双侧兔膝关节胶原诱导关节炎模型(AIA模型),将85只造模成功兔随机分入不同超声峰值负压、不同脉冲宽度、不同脉冲重复频率、不同超声辐照时间、不同微泡浓度、不同辐照次数组,经兔耳缘静脉推注微泡Sonovue,同时超声辐照右侧膝关节,在超声辐照1d后处死,根据MRI及病理结果选出最适合的超声空化条件。结果超声空化效应破坏滑膜血管翳最适合的条件为间歇式超声辐照,间歇时间6s,超声峰值负压4.6MPa,脉冲宽度100cycles,脉冲重复频率50Hz,超声辐照时间5min,微泡浓度0.6mL/kg,多次超声辐照。辐照后右侧膝关节MRI显示滑膜厚度较左侧变薄,病理可见滑膜坏死。结论在适合的超声辐照及微泡浓度条件下,超声空化效应可破坏兔胶原诱导性关节炎滑膜血管翳。
Objective To investigate the optimal ultrasonic irradiation and microbubble conditions of ultrasound-induced cavitation on collagen-induced arthritis synovial pannus in rabbits. Methods Rabbit knee joint collagen-induced arthritis model (AIA model) was established. Eighty successful rabbits were randomly divided into three groups: negative pressure with different ultrasound, different pulse width, different pulse repetition frequency, different ultrasonic irradiation time, different micro At the same time, the right knee of Sonovue was infused by ultrasound through the ear vein of rabbits. One day after irradiation, the mice were sacrificed. The most suitable ultrasound cavitation was selected according to MRI and pathological results condition. Results Ultrasound cavitation effect of synovial pannus is the most suitable conditions for intermittent ultrasound irradiation, intermittent time 6s, ultrasonic peak negative pressure 4.6MPa, pulse width 100cycles, pulse repetition frequency 50Hz, ultrasonic irradiation time 5min, microbubble concentration 0.6mL / kg, multiple ultrasound irradiation. Right knee MRI showed synovial thickness thinner than the left thin, synovial necrosis visible pathology. Conclusions Ultrasound cavitation can damage synovial pannus of collagen-induced arthritis in rabbits under suitable ultrasonic irradiation and microbubble concentration.