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在发展新的半合成蒽环类抗癌抗生素的研制过程中,试用了新近在四川省肿瘤研究所建立的“人体肿瘤材料筛选系统”(HTMSS,Human Tumor Materials Sereening System)——包括人DNA,人癌细胞,人体肿瘤组织三级筛选:1.体外HDI系统(Human DNA Interaction System.):首先从分子水平上提供抗癌筛选信息,快速考察新衍生物与人癌细胞DNA的作用效应及模式,与已知抗癌药物相比较。2.细胞培养试验,运用细胞集落形成能力试验(ColonyFormation Assay,CFA),大分子物质合成试验(Macromolecular Synthesis),DNA转化试验(DNA Transfer Method),骨髓细胞毒性试验,细胞免疫功能试验等方法,从
In the process of developing new semisynthetic anthracycline anti-cancer antibiotics, a new “Human Tumor Materials Screening System” (HTMSS), which was established recently by the Sichuan Institute of Oncology, was used, including human DNA, Human cancer cells, human tumor tissue three screening: 1. In vitro HDI system (Human DNA Interaction System.): First of all, from the molecular level to provide anti-cancer screening information to quickly examine the new derivatives and human cancer cells DNA effect and mode , Compared with known anti-cancer drugs. Cell culture assays were performed using methods such as Colony Formation Assay (CFA), Macromolecular Synthesis, DNA Transfer Method, Bone Marrow Cytotoxicity Test and Cellular Immune Function Test, From