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目的建立RP-HPLC法同时测定毛冬青根中女贞子苷、橄榄苦苷、oleoacteoside和ligstroside共4种环烯醚萜类化合物的含量。方法采用Kromasil C18柱(250 mm×4.60 mm,5μm),以乙腈-质量分数为0.1%的磷酸水溶液为流动相梯度洗脱,流速为1.0 mL·min~(-1),柱温为30℃,检测波长为230 nm,进样量为10μL。结果女贞子苷、橄榄苦苷、oleoacteoside和ligstroside与其邻近的色谱峰均能良好的分离,进样量分别在0.061 35~1.227 0μg(r=0.999 6)、0.294 75~5.895 0μg(r=0.999 5)、0.064 00~1.280 0μg(r=0.999 5)和0.049 76~0.995 2μg(r=0.999 7)内与峰面积呈良好的线性关系,平均加样回收率分别为99.0%(RSD=1.8%)、99.6%(RSD=1.8%)、98.9%(RSD=1.2%)和100.3%(RSD=1.8%)。结论该法准确、可靠,为毛冬青根中环烯醚萜类成分的开发和应用提供参考。
OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of four iridoid compounds of Ligustri lucidum, Oleuropein, oleoacteoside and ligstroside in Mao dongqing root. Methods Kromasil C18 column (250 mm × 4.60 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid. The flow rate was 1.0 mL · min -1 and the column temperature was 30 ℃ , Detection wavelength of 230 nm, injection volume of 10μL. Results Ligustrazine, oleuropetinoside and ligstroside were separated well from their neighboring peaks. The injection volumes were 0.061 35-1.272 0 μg (r = 0.999 6), 0.294 75-5.895 0 μg (r = 0.999 5), 0.06400 ~ 1.280 0μg (r = 0.999 5) and 0.04976 ~ 0.995 2μg (r = 0.999 7). The average recoveries were 99.0% (RSD = 1.8% ), 99.6% (RSD = 1.8%), 98.9% (RSD = 1.2%) and 100.3% (RSD = 1.8%). Conclusion The method is accurate and reliable, providing a reference for the development and application of iridoid constituents in the root of.