论文部分内容阅读
本文对自建的人卵巢癌细胞系（ＨＯ－８９１０）和用该细胞建成的高转移人卵巢癌裸鼠皮下移植癌模型（ＮＳＭＯ）进行了细胞遗传学的比较研究。结果提示：两株细胞均属超二倍体核型，众数为５４条。１、３、１５和２２号染色体常见缺失，１０、１６、１９和２２号染色体常见增加，部分核型中Ｘ染色体有１条缺失，同时发现由１、３、１３、１４和１５号染色体异常而形成的４个标记染色体。ＨＯ－８９１０核型不同的特征是除了４个出现频率较高的标记染色体外，还可见一些其他异常染色体，而ＮＳＭＯ核型中只出现与ＨＯ－８９１０同样的４个标记染色体，很少见有其他异常的染色体，这４个标记染色体可能是高转移卵巢癌独特的遗传特征，这对深入探讨卵巢癌病因和癌变机理有其重要意义。
In this paper, a comparative study of cytogenetics between self-constructed human ovarian cancer cell line (HO-8910) and human metastatic human ovarian cancer subcutaneously transplanted tumor model (NSMO) constructed with this cell line was carried out. The results suggest that: the two cells are diploid karyotype, the mode is 54. Chromosomes 1, 3, 15 and 22 were common deletions. Chromosomes 10, 16, 19 and 22 were common. One part of the X chromosome was missing in some karyotypes. Chromosomes 1, 3, 13, 14 and 15 were abnormal While forming four marker chromosomes. The different features of HO-8910 karyotype are that in addition to the four more frequently occurring marker chromosomes, some other abnormal chromosomes can be seen, whereas only four marker chromosomes of HO-8910 are found in the NSMO karyotype, The other abnormal chromosomes, these four marker chromosomes may be the unique genetic characteristics of highly metastatic ovarian cancer, which is of great significance for further exploring the etiopathogenisis and carcinogenesis mechanism of ovarian cancer.