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目的 观察智脑胶囊对实验性AD模型大鼠学习记忆及自由基代谢的影响。方法 采用 β 淀粉样蛋白(β AP)大鼠侧脑室注射 ,联用转移生长因子 β1(TGFβ1)脑内注射 ,制作实验性AD大鼠模型。在此基础上 ,应用避暗法和水迷路法测定AD模型大鼠学习记忆功能 ,并采用黄嘌呤氧化酶法、硫代巴比妥酸比色法分别测定实验性AD模型大鼠脑组织超氧化物歧化酶 (SOD )活性和丙二醛 (MDA)含量。结果 经改进由 β AP联用TGFβ1诱导的AD模型大鼠既表现有行为学改变 (学习记忆障碍 ) ,又有典型的AD病理学特征 (β AP沉积斑 ) ,同时模型大鼠脑组织SOD活性明显下降 ,MDA含量显著增加。智脑胶囊能有效地改善实验性AD模型大鼠学习记忆功能 ,提高模型大鼠脑组织SOD活性 ,减少MDA含量。结论 智脑胶囊能明显提高 β AP联用TGFβ1诱导的实验性AD模型大鼠学习记忆能力 ,其机制与清除自由基密切相关。
Objective To observe the effect of Zhinao Capsule on learning and memory and free radical metabolism in experimental AD model rats. Methods The rat model of AD was established by intracerebroventricular injection of β-amyloid protein (β AP) in combination with transforming growth factor-β1 (TGFβ1). On this basis, the learning and memory function of AD model rats was determined by the method of avoidance and water labyrinth, and the levels of brain ultrastructure in AD model rats were determined by xanthine oxidase method and thiobarbituric acid colorimetry respectively Oxidative dismutase (SOD) activity and malondialdehyde (MDA) content. RESULTS: The AD model rats induced by TGF-β1 and TGFβ1 improved the behavioral changes (learning and memory impairment) and the typical pathological features of AD (β-AP deposition spots), while the activity of SOD in the model rats Significantly decreased, MDA content increased significantly. Zhinao Capsule can effectively improve the learning and memory function of experimental AD model rats, improve the activity of SOD in brain tissue of model rats and reduce the content of MDA. Conclusion Zhinao capsule can obviously improve the learning and memory ability of experimental AP model induced by TGFβ1 in β-AP combined with β-AP, the mechanism of which is closely related to scavenging free radicals.