论文部分内容阅读
目的探讨脑外伤后不同时间给予高压氧治疗对大鼠认知功能的疗效,及对神经可塑性的影响。方法选择30只大鼠作为干预组建立大鼠脑外伤模型,10只作为假手术组。干预组按照造模后1h、2周、4周开始给予高压氧治疗又分为3组,每亚组10只,各进行1个疗程的高压氧治疗。4组大鼠在基线和第8周应用水迷宫测试,测定海马、纹状体的GAP-43、Syn表达水平,比较治疗效果。结果第8周时,干预组逃避潜伏期和目标象限停留时间比例均高于基线;各干预组逃避潜伏期与假手术组相比,差异均有统计学意义(P<0.05);1h组、2周组与4周组逃避潜伏期相比,差异均有统计学意义(P<0.05)。第8周时,各干预组目标象限停留时间比例与假手术组相比,差异均有统计学意义(P<0.05);1 h组、2周组与4周组相比,差异均有统计学意义(P<0.05)。GAP-43、Syn的变化结果见表1。2周组的GAP-43水平与假手术组和1 h组相比,差异均有统计学意义(P<0.05);2周组和4周组的Syn水平与1h组相比,差异均有统计学意义(P<0.05)。结论高压氧治疗的开始时间对远期的认知功能疗效存在一定影响,但并不是越早越好。高压氧治疗的开始时间越晚,可能对神经重塑的促进作用越明显。
Objective To investigate the curative effect of hyperbaric oxygen therapy on cognitive function in rats after traumatic brain injury at different times and its effect on neuroplasticity. Methods Thirty rats were selected as the intervention group to establish the model of traumatic brain injury in rats. Ten rats were used as sham operation group. The intervention group according to the model 1h, 2 weeks, 4 weeks began to give hyperbaric oxygen treatment was divided into 3 groups, 10 in each subgroup, each for a course of hyperbaric oxygen therapy. The rats in 4 groups were subjected to water maze test at baseline and 8 weeks. The levels of GAP-43 and Syn in hippocampus and striatum were measured and the therapeutic effects were compared. Results At the 8th week, the escape latency of the intervention group and the target quadrant retention time were all higher than those of the baseline. The escape latency of each intervention group was significantly higher than that of the sham operation group (P <0.05) Compared with 4 weeks group, the differences were significant (P <0.05). At the 8th week, the difference of the target quadrant stay time in each intervention group was statistically significant compared with that in the sham operation group (P <0.05). The difference was statistically significant between 1 h group, 2 weeks group and 4 weeks group Significance (P <0.05). GAP-43, Syn changes in Table 1.2 weeks GAP-43 levels compared with sham operation group and 1 h group, the difference was statistically significant (P <0.05); 2 weeks and 4 weeks group The Syn level compared with 1h group, the difference was statistically significant (P <0.05). Conclusions The onset time of hyperbaric oxygen therapy has some effect on long-term cognitive efficacy, but not earlier and better. The later start of hyperbaric oxygen therapy, the more likely to promote the role of nerve remodeling.